Cloning 101

Cloning allows you to grow an exact copy of your plant by using the original plant roots. Here is a basic guide to cloning your plants.

What You'll Need...

 
Tray & Dome                             Light                         Growing Medium      
   
          1. On your plant look for a spot where there are branches growing out and a new top, and cut a little bit below that. Cut the branch away at a 45 degree angle. You want probably around 5-8 inches to cut for your new clone. 
          2. Now choose the growing medium you are going to use. There are Jiffy pellets, Rockwool, Rapid Rotter cubes, or loose soilless mix. Pre-soak which growing medium you prefer. Also choose which rooting hormone you prefer- powder or a gel. Give a light coating of either on the stem of your cut and insert it into the medium. 
      1. Arrange your "planted" cuttings in your tray and your dome. The dome will provide humidity and keep heat in. Under the tray it is preferable to put a heating mat. This will increase your chances of rooting. Make sure there is no water in the bottom of the tray.  
      2. On top of your dome you will want to put a propagation light. We recommend the T5HO Sunblaster 6400k light or even better, the 18" Clone LED from Quick Grow Supercenter.                                                                                                                                                                                                                                                                                                                                                                                                                                  
      3. Do Not Spray your cuttings. We are trying to get the clones to want to look for water, in order to do that they must grow roots to find it. If the humidity in the dome is too high they will have sufficient moisture to be able to survive without growing roots. After 5-7 days you will need to water the cuttings. You can remove each one and dunk them in water or you can water in tray, but remember to drain the excess water.                                                             
      4. Within 14 days you should have signs of new roots that signify you can transplant to a new container.

Also FROM THE BLOG...

Aeroponics – Misting Frequency and The Root Systems

Aeroponic systems, which use a mist of nutrients over the plant roots, inside a growing chamber. Producing faster growth rates, high yields and healthy roots. As long as the plant rooting chamber is being kept between 62F – 71F consistently. Some of the more sophisticated commercial greenhouse systems are temperature linked. The temperature is continually monitored in the root chambers, when pre-set temperature is triggered the mister system is activated to bring temperatures down.

Simple Misting Time

One method of delivering nutrient spray in commercial aeroponic systems is the ‘regular, intermittent misting cycle’. This is a burst of nutrient solution, misting 3 minutes every 5 minutes. By using this technique, which does not change during the life of the crop, the misting cycle never causes the plant’s roots to dry out. The emphasis here, is on regularly delivery fresh aerated, temperature adjusted nutrient to the root zone.

Continual Misting with Proper “Conditions”

With proper oxygen and temperature ( 62F – 71F ) in the nutrient solution in the aeroponic growing chamber, the plant root system will not become water logged or root rot problems. The plants root system on continual misting cycle will produces extremely healthy roots and high yields of plant material. Continual misting eliminates the problems of roots drying out between misting cycles and is one way of ensuring temperatures in the root zone stay stable and do not fluctuate.

The Need for Tweaking

Aeroponic timers allows the grower ability to adjust the frequency of the on/off misting or spraying cycle as well as how long the roots are misted for. FHD has discovered that by changing the cycle timer during the plant stages of life, we received overall better production without adding higher cost in the systems. This idea is based on applying more oxygen to the root system than continual misting cycle. When using this type of system the following points should be taken into account.

1. There is not one set ideal misting program, the amount of nutrient mist time required, is largely depended on the plant, stage of development and more importantly the temperature in the root chamber during the plant stages.

2. Each growing environment is different. The need for experimenting is crucial in receiving eXtreme harvest. Take your time, set your timer 1 minute on and 1 minute off. Then watch the program in action allowing to repeat its self a few times making sure the plant leaves don’t start to wilt from lack of nutrient mist. If no sign of wilting, increase off time for a minute. Continue until desired setting is reached or 10 minutes is reached. Repeat this programming once a week for that growing week. Ultimate would be 1 minute on and 2 minutes off, for first 2 weeks of vegetative stage. Then moving to a 1 minute on and 3 minutes off after shading the growing chamber and the whole duration of flowering a 1 minute on and 10 minutes off.

4. The major benefit of an adjustable misting program is its flexibility in the growing stages of the plant. When propagated in an aeroponic chamber, newly clipped clones need to be constantly misted until rooted with a dome on top to trap humidity to the plant leaves. Once rooted, the root system needs nutrients. The nutrient interval cycles are determined in vegetative and flowering stages by root temperature. As the plant matures, the plant leaves will begin to shadow the growing chamber, reducing temperature, allowing decreasing misting time. By utilizing this procedure, the plant is allowed more oxygen intake to the root hair between feedings, achieving faster and bushier growth. In flowering, the importance of oxygen intake to the root system is staggering. Plants will go from looking beautiful to looking sick and death is inevitable from oxygen starvation.

5. Always keep a close eye on the root system inside an aeroponic chamber – even slight drying of a portion of the root system will result in tissue damage and could lead to pathogen attack.

6. Make sure to use a quality sediment free nutrient, as it’s very important not to have a mister plug up. Remember that in aeroponics, the ppm (EC) in the nutrient solution needs to be less concentrated, than other soil-less systems as the roots intakes the nutrients much more easier.

Nutrient Uptake – Day and Night

Most plants take up nutrients by both day and night. With night time being the more dominant side. Commercial hydroponic growers of ‘heavy feeder’ crops such as cucumbers and tomatoes, experience higher nutrient uptake in the evening and into the night as the temperatures cool down the plants are able to take up more water and nutrients through increased root pressure and more suitable environmental conditions. Warmer conditions during the day, the plant will shut down photosynthesis and transpiration and thus reduce nutrient uptake, and will then feed rapidly in the evening as conditions become cooler. Calcium is taken up during the night when root pressure allows more water uptake and transpiration within the plant, carrying with it calcium into plant tissue.

The Root System

Plant roots, which end up continually submerged in a ‘deep flow’ or constant drip systems will commonly be long, thin, relatively unbranched, yellowing or brown in color and seem to be lacking in fine, fluffy root hairs. The roots which develop up above the flow or pond of nutrient with a mist are typically whiter in color, more branched out and often contain masses of very fluffy, fine, bright white root hairs.

Oxygenation, Air Pumps, Nutrient Uptake and Temperatures

Introduction: Why plant roots need oxygen

Oxygen is an essential plant nutrient – plant root systems require oxygen for aerobic respiration, an essential plant process that releases energy for root growth and nutrient uptake. In many ‘solution culture’ hydroponic systems, the oxygen supplied for plant root uptake is provided mostly as dissolved oxygen (DO) held in the nutrient solution. If depletion of this dissolved oxygen in the root system occurs, then growth of plants, water and mineral uptake are reduced.

Injury from low (or no) oxygen in the root zone can take several forms and these will differ in severity between plant types. Often the first sign of inadequate oxygen supply to the roots is wilting of the plant under warm conditions and high light levels. Insufficient oxygen reduces the permeability of the roots to water and there will be an accumulation of toxins, so that both water and minerals are not absorbed in sufficient amounts to support plant growth. This wilting is accompanied by slower rates of photosynthesis and carbohydrate transfer, so that over time, plant growth is reduced and yields are affected. If oxygen starvation continues, mineral deficiencies will begin to show, roots die back and plants will become stunted. If the lack of oxygen continues in the root zone, plants produce a stress hormone – ethylene, which accumulates in the roots and causes collapse of the root cells, at this stage pathogens such as pythium can easily take hold and destroy the plant.

Oxygen in Hydroponic Nutrient Solutions

While it’s possible to measure the levels of dissolved oxygen in a hydroponic nutrient solution, it’s not carried out as often as EC and pH monitoring due to the cost of accurate DO (Dissolved Oxygen meters). However, if an effective method of aeration is continually being used, and solution temperatures are not reaching excessively high levels, then good levels of oxygenation in most systems can be achieved One of the most common and effective methods of oxygenation in hydroponic nutrient solutions is with the use of air pumps/machines and air stones.

Air Pumps and Air Stones

While there are a number of methods that can be used to introduce oxygen into a nutrient solution, many of these, such as ozone treatment, are expensive and not often used by smaller growers. One of the most practical and inexpensive, yet efficient ways of getting more dissolved oxygen into a plants root system is through forcing air into the nutrient. Air pumps are widely available in a range of sizes, from very small up to very large with capacity to run from one to many `air stones’ each introducing hundreds of tiny bubbles of fresh, oxygen rich air into the nutrient solution.

Why an Air Stone

While an air pump tube alone can bubble air into a nutrient solution, oxygenation or the process of getting atmospheric oxygen dissolved into the liquid nutrient, is much more effective where many tiny bubbles of air are created, rather than a slow stream of larger bubbles. The greater the surface contact between the air bubbles and the nutrient, the more oxygen will diffuse into the nutrient solution and smaller bubbles create a far greater surface area than a few larger bubbles will. Air stones simply break up the air flow and distribute along the surface of the porous ‘stone’ so that many tiny bubbles are rapidly introduced into the nutrient. Depending on the size or dimensions of the nutrient reservoir into which air is being introduced for oxygenation, air stones of different shapes and sizes can be selected. For small rectangular tanks, long thin air stones (some up to 1 foot in length) can be placed on the base of the reservoir to distribute air bubbles and oxygen uniformly. A larger number of smaller, round, cylindrical or oval air stones placed at equal distance inside a nutrient pool or tank also ensure high levels of oxygenation.

Air stones also have the benefit of acting as ‘weights’ which remain stable on the base, or in the lower layers of the nutrient tank – the further the bubbles have to travel to reach the surface of the nutrient, the more time oxygen has to diffuse into the liquid and the higher the rates of dissolved oxygen than can be obtained from an air pump and stone set up.

For systems with multiple nutrient reservoirs or tanks, one large air pump with many outlets will allow oxygenation into all systems and it is always a good idea to buy an air machine and air stones larger than currently required so that aeration can be increased under warmer conditions or if the hydroponic system is later expanded.

Oxygen and Temperature Effects – Effective Aeration

While forcing air bubbles deep down into the nutrient reservoir generally increases the dissolved oxygen levels in the nutrient, there is one other major factor to consider and that’s the temperature of the air being pumped into the nutrient. As the temperature of a nutrient solution increases, its ability to hold dissolved oxygen decreases. So a cool nutrient solution may in fact hold twice as much oxygen at ‘saturation level’ than a warm solution. For example a nutrient solution at 45 F can hold around 12ppm of dissolved oxygen at ‘saturation’, (meaning it is the most it can hold), but the same nutrient solution at a temperature of 85 F will hold less than 7ppm at saturation. This means at a solution temperature of 85F there is much less dissolved oxygen available for the plant’s root system to take up. To complicate matters further, the requirement of the plant’s root system for oxygen at warmer temperatures, is many times greater than at cooler temperatures due to the increased rate of root respiration. So warm nutrients mean a very high oxygen requirement from the plant’s roots, but the nutrient can only hold very limited amounts of dissolved oxygen at saturation, no matter how much air is being bubbled into the solution. Ideally, nutrient solution temperatures for most plants should be run lower than the overall air temperature – this has many beneficial effects on plant growth and development. However, if overly warm air from the growing environment is pumped into an otherwise cool nutrient solution, the warm air will rapidly increase the temperature of the nutrient to that of the growing environment. If air is being pumped via an air machine with an intake close to lights or other heat sources then rapid heating of the nutrient will occur. On the other hand, cool air has the ability to reduce the temperature of the nutrient if sufficient levels are pumped in and thus result in a much more highly oxygenated solution for the plant’s roots. If keeping the nutrient solution temperature down seems to be a continual problem, checking the air inlet temperature of an air pump is a good idea. Overly warm nutrient solutions (ideally nutrient solutions should remain below 65 – 75 F) for most warm season, high light plants and well below 69 F for cool season.can have serious effects on the plants root system. Apart from the increased oxygen requirement due to a much higher rate of root respiration which can rapidly result in oxygen starvation, high solution temperatures favour many of the root disease pathogens. Plant roots become highly ‘stressed’ when experiencing high temperatures, particularly if there is a large mis-match between the air the root temperature. Root stress slows the development of new roots, resulting in reserves inside the root tissue being `burned up’ during respiration faster than they are accumulated, and stress makes the root system in general more susceptible to disease attack. Keeping a check on nutrient temperature is vital, as is ensuring that air machines are not blasting hot air into the solution and cooking plant roots. Aeration is most effective when cool air is bubbled into a nutrient.

Oxygenation and Nutrient Uptake

Healthy roots supplied with sufficient oxygen are able to absorb nutrient ions selectively from the surrounding solution as required. The metabolic energy which is required to drive this nutrient uptake process is obtained from root respiration using oxygen. In fact there can be a net loss of nutrient ions from a plant’s root system when suffering from a lack of oxygen (anaerobic conditions). Without sufficient oxygen in the root zone, plants are unable to take up mineral nutrients in the concentrations required for maximum growth and development. Maintain maximum levels of dissolved oxygen boosts nutrient uptake by ensuring healthy roots have the energy required to rapidly take up and transport water and mineral ions.

Calcium is one important nutrient ion which has been shown to benefit from high levels of oxygenation in the hydroponic nutrient solution Calcium, unlike the other major nutrients is absorbed mostly by the root growing tips (root apex). The root apex has a large energy requirement for new cell production and growth and is therefore vulnerable to oxygen stress If root tips begin to suffer from a lack of oxygen, a shortage of calcium in the shoot will occur. This shortage of calcium makes the development of calcium disorders such as tip burn and blossom end rot of fruit more likely and severe under oxygen starvation conditions. High levels of oxygenation ensure healthy root tips are able to take the levels of calcium required for new tissue growth and development.

Conclusion

While providing oxygenation with the use of air machines and stones is an excellent method of increasing the dissolved oxygen (DO) levels in a nutrient solution, the temperature of the air intake and nutrient solution must also be managed to ensure oxygen starvation in the root zone does not occur. Pumping hot air into a nutrient not only creates temperature stress in the root zone, it also results in less oxygen carrying capacity in the solution itself – a recipe for root suffocation that will rapidly affect the top portion of the plant as well. Getting oxygenation right means checking both aeration capacity of the equipment being chosen and temperatures in the nutrient and root zone.